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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Harmonized reference ranges for Total and Free T levels
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<blockquote data-quote="madman" data-source="post: 218715" data-attributes="member: 13851"><p><strong>Fig. 2. <u>Schematic depiction of the principles of common analytical assays used for the measurement of testosterone concentrations</u>: (A) competitive binding immunoassay, (B) aptamer (synthetic ligand-binding oligonucleotides)-based assay, (C) tracer analog assay for free testosterone, and (D) equilibrium dialysis method for free testosterone. In the equilibrium dialysis method, human serum or plasma is dialyzed against a buffer with the ionic composition of human serum or plasma across a semipermeable membrane. At equilibrium, the dialyzed testosterone fraction can be measured directly by LC-MS/MS, or a radiotracerlabeled testosterone is added to the sample and the dialyzed fraction can be counted to obtain an estimate of free testosterone.</strong></p><p><strong>[ATTACH=full]20031[/ATTACH]</strong></p><p><strong>[ATTACH=full]20032[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 218715, member: 13851"] [B]Fig. 2. [U]Schematic depiction of the principles of common analytical assays used for the measurement of testosterone concentrations[/U]: (A) competitive binding immunoassay, (B) aptamer (synthetic ligand-binding oligonucleotides)-based assay, (C) tracer analog assay for free testosterone, and (D) equilibrium dialysis method for free testosterone. In the equilibrium dialysis method, human serum or plasma is dialyzed against a buffer with the ionic composition of human serum or plasma across a semipermeable membrane. At equilibrium, the dialyzed testosterone fraction can be measured directly by LC-MS/MS, or a radiotracerlabeled testosterone is added to the sample and the dialyzed fraction can be counted to obtain an estimate of free testosterone. [ATTACH type="full"]20031[/ATTACH] [ATTACH type="full"]20032[/ATTACH][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Harmonized reference ranges for Total and Free T levels
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