ExcelMale
Menu
Home
What's new
Latest activity
Forums
New posts
Search forums
What's new
New posts
Latest activity
Videos
Lab Tests
Doctor Finder
Buy Books
About Us
Men’s Health Coaching
Log in
Register
What's new
Search
Search
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Navigation
Install the app
Install
More options
Contact us
Close Menu
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Clomid for PCT, fertility or low T
FSHB and FSHR genetic variants and testicular function
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Message
<blockquote data-quote="madman" data-source="post: 191594" data-attributes="member: 13851"><p><strong>FSHB and FSHR gene variants exert mild modulatory effect on reproductive hormone levels and testis size but not on semen quality: A study of 2,020 men from the general Danish population </strong></p><p><span style="color: rgb(44, 130, 201)">Anne Kirstine Bang, Kristian Almstrup, Loa Nordkap, Lærke Priskorn, Jørgen Holm Petersen, Martin Blomberg Jensen, Marianna Krause, Stine Agergaard Holmboe, Dorte Louise Egeberg Palme, Sofia Boeg Winge, Ulla Nordström Joensen, Inge Ahlmann Olesen, Helene Westring Hvidman, Anders Juul, Ewa Rajpert-De Meyts, Niels Jørgensen</span></p><p></p><p></p><p></p><p></p><p><strong>Abstract </strong></p><p><strong></strong></p><p><strong>Background:</strong> <span style="color: rgb(184, 49, 47)"><em>Spermatogenesis depends on stimulation by follicle-stimulating hormone (FSH) which binds to FSH receptors (FSHR) on testicular Sertoli cells.</em></span> <span style="color: rgb(44, 130, 201)"><em>Three FSH-related single nucleotide polymorphisms (SNPs); FSHB -211G>T (rs10835638), FSHR -29G>A (rs1394205), and FSHR 2039A>G (rs6166) affect FSH action and have been suggested to affect testicular function, but the evidence is uncertain. </em></span></p><p></p><p><strong>Objective:</strong> <span style="color: rgb(184, 49, 47)"><em>To describe the associations between the three SNPs and testicular function in a large and well-characterized cohort of men from the general population. </em></span></p><p></p><p><strong>Materials and methods:</strong> A cross-sectional study of 2,020 Danish men unselected regarding testicular function.</p><p>Outcome variables were semen parameters, reproductive hormones, and testis size. Genotyping was done by competitive allele-specific quantitative PCR. Differences in genotype frequencies were tested by Chi-square test and associations between genotypes and outcomes were assessed by multivariate linear regressions.</p><p></p><p><strong>Results:</strong> The SNPs affected serum FSH; carriers of the variant affecting FSH secretion (FSHB - 211G>T) had lower FSH levels while carriers of variants affecting receptor expression (FSHR - 29G>A) and receptor sensitivity (FSHR 2039A>G) had higher FSH levels. Carriers of FSHB - 211G>T had a lower calculated free-Testosterone/LH ratio. Although both FSHB -211G>T and FSHR 2039A>G was associated with smaller testis size, no clear association was detected in relation to any semen parameters, except a lower total number of morphologically normal spermatozoa in the heterozygous carriers of the FSHB -211G>T Discussion and</p><p></p><p><strong>Conclusion:</strong> <span style="color: rgb(184, 49, 47)"><em>The studied polymorphisms have only a minor modulating influence on testis size and function in healthy men. </em></span><span style="color: rgb(44, 130, 201)"><em>We detected subtle effects of the three SNPs on FSH levels, but also effects of FSHB -211G>T on calculated free-Testosterone/LH ratio, compatible with altered Leydig cell function. </em></span><span style="color: rgb(184, 49, 47)"><em>Thus, the role of these FSH-related polymorphisms is complex and modest in men with normal testicular function, but the possible importance of FSH polymorphisms in men with impaired testicular function should be evaluated in future studies in more detail. </em></span></p><p></p><p></p><p></p><p></p><p></p><p><strong>Introduction </strong></p><p></p><p><em><span style="color: rgb(184, 49, 47)">Spermatogenesis can be affected by multiple factors including genetic variations modulating the function of the hypothalamus-pituitary-gonadal (HPG) hormone axis1,2 as well as environmental and lifestyle factors3,4.</span></em></p><p><em><span style="color: rgb(184, 49, 47)"></span></em></p><p><em><span style="color: rgb(184, 49, 47)">Single nucleotide polymorphisms (SNPs) in several genes have been associated with male infertility and decreased semen quality1,2. </span><span style="color: rgb(44, 130, 201)">SNPs that affect the follicle-stimulating hormone (FSH) signaling pathway essential for normal spermatogenesis, have gained attention5–9. Particularly, the FSHB -211G>T SNP, located in the promoter region of the FSH beta-subunit, influences the transcription of the FSHB gene and consequently circulating FSH levels10. Minor T-allele carriers of FSHB -211G>T have lower serum levels of FSH and inhibin B and smaller testicular volume10– 15. </span><span style="color: rgb(184, 49, 47)">Furthermore, two FSH receptor (FSHR) SNPs, FSHR -29G>A and FSHR 2039A>G, have been linked to testicular function by affecting the transcription of FSHR and the sensitivity of FSHR, respectively14–19. </span><span style="color: rgb(44, 130, 201)">In some studies, the minor alleles of these three SNPs were found to be more prevalent among infertile men than among men from the general populations and proven fathers11,13,20, the FSHR SNPs in combination with the FSHB -211G>T SNP have been shown to result in a more severe phenotype14.</span><span style="color: rgb(184, 49, 47)"> However, other studies found no effect of especially the FSHR variants on male reproduction suggesting that the HPG axis may compensate for the effects of these genetic variants in some men9,21. </span><span style="color: rgb(44, 130, 201)">Here, we address this question by investigating the three most informative SNPs: FSHB -211G>T, FSHR -29G>A, and FSHR 2039A>G on gonadal function, in a very large group of well-characterized young Danish men from the general population.</span></em></p><p><em></em></p><p><em></em></p><p><em></em></p><p><em></em></p><p><em></em></p><p><em><strong><span style="color: rgb(184, 49, 47)">In conclusion, our study investigated the combined effects of three FSH-related SNPs. The findings of the present study are overall in agreement with previously published studies. Our results do not support a major impact of the FSH-related SNPs on testicular function in men with largely normal testis function.</span></strong></em> <em><strong><span style="color: rgb(44, 130, 201)">A minor modulating influence of the genotype distribution on the average parameters of the testis function at the population level is possible, though at the individual level the effects of these genotypes on testis function are apparently subtle and likely influenced by other factors in healthy young men. </span></strong></em></p></blockquote><p></p>
[QUOTE="madman, post: 191594, member: 13851"] [B]FSHB and FSHR gene variants exert mild modulatory effect on reproductive hormone levels and testis size but not on semen quality: A study of 2,020 men from the general Danish population [/B] [COLOR=rgb(44, 130, 201)]Anne Kirstine Bang, Kristian Almstrup, Loa Nordkap, Lærke Priskorn, Jørgen Holm Petersen, Martin Blomberg Jensen, Marianna Krause, Stine Agergaard Holmboe, Dorte Louise Egeberg Palme, Sofia Boeg Winge, Ulla Nordström Joensen, Inge Ahlmann Olesen, Helene Westring Hvidman, Anders Juul, Ewa Rajpert-De Meyts, Niels Jørgensen[/COLOR] [B]Abstract Background:[/B] [COLOR=rgb(184, 49, 47)][I]Spermatogenesis depends on stimulation by follicle-stimulating hormone (FSH) which binds to FSH receptors (FSHR) on testicular Sertoli cells.[/I][/COLOR] [COLOR=rgb(44, 130, 201)][I]Three FSH-related single nucleotide polymorphisms (SNPs); FSHB -211G>T (rs10835638), FSHR -29G>A (rs1394205), and FSHR 2039A>G (rs6166) affect FSH action and have been suggested to affect testicular function, but the evidence is uncertain. [/I][/COLOR] [B]Objective:[/B] [COLOR=rgb(184, 49, 47)][I]To describe the associations between the three SNPs and testicular function in a large and well-characterized cohort of men from the general population. [/I][/COLOR] [B]Materials and methods:[/B] A cross-sectional study of 2,020 Danish men unselected regarding testicular function. Outcome variables were semen parameters, reproductive hormones, and testis size. Genotyping was done by competitive allele-specific quantitative PCR. Differences in genotype frequencies were tested by Chi-square test and associations between genotypes and outcomes were assessed by multivariate linear regressions. [B]Results:[/B] The SNPs affected serum FSH; carriers of the variant affecting FSH secretion (FSHB - 211G>T) had lower FSH levels while carriers of variants affecting receptor expression (FSHR - 29G>A) and receptor sensitivity (FSHR 2039A>G) had higher FSH levels. Carriers of FSHB - 211G>T had a lower calculated free-Testosterone/LH ratio. Although both FSHB -211G>T and FSHR 2039A>G was associated with smaller testis size, no clear association was detected in relation to any semen parameters, except a lower total number of morphologically normal spermatozoa in the heterozygous carriers of the FSHB -211G>T Discussion and [B]Conclusion:[/B] [COLOR=rgb(184, 49, 47)][I]The studied polymorphisms have only a minor modulating influence on testis size and function in healthy men. [/I][/COLOR][COLOR=rgb(44, 130, 201)][I]We detected subtle effects of the three SNPs on FSH levels, but also effects of FSHB -211G>T on calculated free-Testosterone/LH ratio, compatible with altered Leydig cell function. [/I][/COLOR][COLOR=rgb(184, 49, 47)][I]Thus, the role of these FSH-related polymorphisms is complex and modest in men with normal testicular function, but the possible importance of FSH polymorphisms in men with impaired testicular function should be evaluated in future studies in more detail. [/I][/COLOR] [B]Introduction [/B] [I][COLOR=rgb(184, 49, 47)]Spermatogenesis can be affected by multiple factors including genetic variations modulating the function of the hypothalamus-pituitary-gonadal (HPG) hormone axis1,2 as well as environmental and lifestyle factors3,4. Single nucleotide polymorphisms (SNPs) in several genes have been associated with male infertility and decreased semen quality1,2. [/COLOR][COLOR=rgb(44, 130, 201)]SNPs that affect the follicle-stimulating hormone (FSH) signaling pathway essential for normal spermatogenesis, have gained attention5–9. Particularly, the FSHB -211G>T SNP, located in the promoter region of the FSH beta-subunit, influences the transcription of the FSHB gene and consequently circulating FSH levels10. Minor T-allele carriers of FSHB -211G>T have lower serum levels of FSH and inhibin B and smaller testicular volume10– 15. [/COLOR][COLOR=rgb(184, 49, 47)]Furthermore, two FSH receptor (FSHR) SNPs, FSHR -29G>A and FSHR 2039A>G, have been linked to testicular function by affecting the transcription of FSHR and the sensitivity of FSHR, respectively14–19. [/COLOR][COLOR=rgb(44, 130, 201)]In some studies, the minor alleles of these three SNPs were found to be more prevalent among infertile men than among men from the general populations and proven fathers11,13,20, the FSHR SNPs in combination with the FSHB -211G>T SNP have been shown to result in a more severe phenotype14.[/COLOR][COLOR=rgb(184, 49, 47)] However, other studies found no effect of especially the FSHR variants on male reproduction suggesting that the HPG axis may compensate for the effects of these genetic variants in some men9,21. [/COLOR][COLOR=rgb(44, 130, 201)]Here, we address this question by investigating the three most informative SNPs: FSHB -211G>T, FSHR -29G>A, and FSHR 2039A>G on gonadal function, in a very large group of well-characterized young Danish men from the general population.[/COLOR] [B][COLOR=rgb(184, 49, 47)]In conclusion, our study investigated the combined effects of three FSH-related SNPs. The findings of the present study are overall in agreement with previously published studies. Our results do not support a major impact of the FSH-related SNPs on testicular function in men with largely normal testis function.[/COLOR][/B][/I] [I][B][COLOR=rgb(44, 130, 201)]A minor modulating influence of the genotype distribution on the average parameters of the testis function at the population level is possible, though at the individual level the effects of these genotypes on testis function are apparently subtle and likely influenced by other factors in healthy young men. [/COLOR][/B][/I] [/QUOTE]
Insert quotes…
Verification
Post reply
Share this page
Facebook
Twitter
Reddit
Pinterest
Tumblr
WhatsApp
Email
Share
Link
Sponsors
Forums
Testosterone Replacement, Low T, HCG, & Beyond
Clomid for PCT, fertility or low T
FSHB and FSHR genetic variants and testicular function
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.
Accept
Learn more…
Top