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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Free Testosterone & cardiometabolic parameters in men - comparison of algorithms
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<blockquote data-quote="madman" data-source="post: 195396" data-attributes="member: 13851"><p>*In addition to baseline levels of metabolic parameters, our follow-up study made it possible to also investigate long-term cardiovascular outcomes. <strong><em><u>Some limitations should however be considered when interpreting the results</u>. </em></strong>The aim of the study was to compare different algorithms of free testosterone in relation to metabolic parameters.<strong><em> <u>It would, however, have been relevant also to include direct measurements of free testosterone using equilibrium dialysis to directly compare the different algorithms to the gold standard method</u>. <u>Due to lack of serum, this was however not a possibility</u>. </em></strong><em>Furthermore, no information on symptoms of androgen insufficiency was available. Thus, a comparison of the three free testosterone algorithms in relation to androgen activity was not possible. Likewise, information on other measures of biological activity of testosterone, such as PSA levels or hematocrit levels, were not available. Information on LH, the primary stimulator of testosterone was however available but the calculated ratio between LH and the algorithms by Vermeulen et al. and Zakharov et al did not reveal any differences in relation to the included survival outcomes (data not shown).</em><strong><em> <u>Another limitation in the study is the lack of measurements of albumin levels which is included for calculation of free testosterone, because of limitations of available serum volume</u>.<u> However, as relatively large changes in albumin is needed to significantly affect the calculated free testosterone level we used a fixed average level of albumin in both the algorithm by Vermeulen et al. and Zakharov et al</u>. <u>Both testosterone and SHBG were measured using immunoassays</u>. <u>Measurements of sex steroids by use of immunoassays have been criticized for being less reliable compared to methods based on liquid or gas chromatography-tandem mass spectrometry, which today are recommended for analyses of small molecules like steroid hormones</u> (1, 33). </em></strong><em>The main problem of immunoassays relates to their tendency of overestimating concentrations in the lower range but this issue is less significant in higher measurement ranges (1, 34). Thus, this is not a major concern in this study based on adult men from the general population.</em></p></blockquote><p></p>
[QUOTE="madman, post: 195396, member: 13851"] *In addition to baseline levels of metabolic parameters, our follow-up study made it possible to also investigate long-term cardiovascular outcomes. [B][I][U]Some limitations should however be considered when interpreting the results[/U]. [/I][/B]The aim of the study was to compare different algorithms of free testosterone in relation to metabolic parameters.[B][I] [U]It would, however, have been relevant also to include direct measurements of free testosterone using equilibrium dialysis to directly compare the different algorithms to the gold standard method[/U]. [U]Due to lack of serum, this was however not a possibility[/U]. [/I][/B][I]Furthermore, no information on symptoms of androgen insufficiency was available. Thus, a comparison of the three free testosterone algorithms in relation to androgen activity was not possible. Likewise, information on other measures of biological activity of testosterone, such as PSA levels or hematocrit levels, were not available. Information on LH, the primary stimulator of testosterone was however available but the calculated ratio between LH and the algorithms by Vermeulen et al. and Zakharov et al did not reveal any differences in relation to the included survival outcomes (data not shown).[/I][B][I] [U]Another limitation in the study is the lack of measurements of albumin levels which is included for calculation of free testosterone, because of limitations of available serum volume[/U].[U] However, as relatively large changes in albumin is needed to significantly affect the calculated free testosterone level we used a fixed average level of albumin in both the algorithm by Vermeulen et al. and Zakharov et al[/U]. [U]Both testosterone and SHBG were measured using immunoassays[/U]. [U]Measurements of sex steroids by use of immunoassays have been criticized for being less reliable compared to methods based on liquid or gas chromatography-tandem mass spectrometry, which today are recommended for analyses of small molecules like steroid hormones[/U] (1, 33). [/I][/B][I]The main problem of immunoassays relates to their tendency of overestimating concentrations in the lower range but this issue is less significant in higher measurement ranges (1, 34). Thus, this is not a major concern in this study based on adult men from the general population.[/I] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
Free Testosterone & cardiometabolic parameters in men - comparison of algorithms
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