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Congratulations!

Definitely a big step to take as it can be an ongoing struggle for many.

Much better for your overall health in the long run.

Highly doubtful there would be any impact on your TT, FT, and estradiol levels from the nicotine.

You most likely feel off due to going through the withdrawal.

If anything I would be more concerned with your diet/appetite after quitting as many tend to gain weight and unfortunately it is adipose tissue that can have a big impact on estradiol.

If you start packing on the blubber it will have a negative impact on your hormones let alone other blood markers.