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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Difference between ECLIA (Regular) vs LC/MS (Sensitive) Estradiol Test Methods
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<blockquote data-quote="Nelson Vergel" data-source="post: 161974" data-attributes="member: 3"><p><span style="font-size: 18px"><span style="font-family: 'Arial'">I found an interesting paper that may help men outside the US where the LC/MS sensitive estradiol test is not available (attached file for registered members)</span></span></p><p></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'"><strong>Comparison of serum testosterone and estradiol measurements</strong></span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'"><strong>in 3174 European men using platform immunoassay (IA) and mass</strong></span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'"><strong>spectrometry; relevance for the diagnostics in aging men</strong></span></span></p><p></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">Abstract</span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">Background: The limitations of serum testosterone and estradiol (E2) measurements using non extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce.</span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">Methods: We compared serum testosterone and E2measurements in a large sample of middle-aged/elderly men using a common platform IA and gas chromatography (GC)–MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European MaleAging Study (nZ3174; age 40–79 years), peripheral serum testosterone and E2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC–MS methods.</span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">Results: Over a broad concentration range, serum testosterone concentration measured by IA and MSshowed high correlation, which was less robust in the hypogonadal range. The IA/MS correlation was weaker in E2 measurements. Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism(<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E2</span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">(!40.7 pmol/l) were 13.3 and 99.3%, and for high E2 (>120 pmol/l) 88.4 and 88.6%.</span></span></p><p><span style="font-size: 18px"><span style="font-family: 'Arial'">Conclusion: A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism.<strong> The used for E2 measurements showed poor correlation with MS and may only be suitable for the detection of high E2 in men.</strong></span></span></p><p></p><p></p><p><em><span style="font-size: 18px">European Journal of Endocrinology 166 983–991</span></em></p><p></p><p><span style="font-size: 18px"><span style="color: rgb(226, 80, 65)">It seems that the mean difference between the two E2 tests was 30% (higher for IA test)</span></span></p><p></p><p>[ATTACH=full]8561[/ATTACH]</p><p></p><p>[ATTACH=full]8562[/ATTACH]</p></blockquote><p></p>
[QUOTE="Nelson Vergel, post: 161974, member: 3"] [SIZE=18px][FONT=Arial]I found an interesting paper that may help men outside the US where the LC/MS sensitive estradiol test is not available (attached file for registered members)[/FONT][/SIZE] [SIZE=18px][FONT=Arial][B]Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay (IA) and mass spectrometry; relevance for the diagnostics in aging men[/B][/FONT][/SIZE] [SIZE=18px][FONT=Arial]Abstract Background: The limitations of serum testosterone and estradiol (E2) measurements using non extraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce. Methods: We compared serum testosterone and E2measurements in a large sample of middle-aged/elderly men using a common platform IA and gas chromatography (GC)–MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European MaleAging Study (nZ3174; age 40–79 years), peripheral serum testosterone and E2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC–MS methods. Results: Over a broad concentration range, serum testosterone concentration measured by IA and MSshowed high correlation, which was less robust in the hypogonadal range. The IA/MS correlation was weaker in E2 measurements. Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism(<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E2 (!40.7 pmol/l) were 13.3 and 99.3%, and for high E2 (>120 pmol/l) 88.4 and 88.6%. Conclusion: A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism.[B] The used for E2 measurements showed poor correlation with MS and may only be suitable for the detection of high E2 in men.[/B][/FONT][/SIZE] [I][SIZE=18px]European Journal of Endocrinology 166 983–991[/SIZE][/I] [SIZE=18px][COLOR=rgb(226, 80, 65)]It seems that the mean difference between the two E2 tests was 30% (higher for IA test)[/COLOR][/SIZE] [ATTACH=full]8561[/ATTACH] [ATTACH=full]8562[/ATTACH] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Difference between ECLIA (Regular) vs LC/MS (Sensitive) Estradiol Test Methods
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