Guess this went over your noggin too!
* Limitations of using free testosterone by equilibrium dialysis and calculated free testosterone concentrations in practice are the lack of assay standardization, an accuracy-based quality control program, and a harmonized reference range. Until these limitations are addressed, free testosterone by equilibrium dialysis and calculated free testosterone should use reference ranges established by individual laboratories or their specific assay method
Again for the last time!
My reply to previous threads:
When it comes to blood work whether one is following a strictly sub-q protocol, strictly IM protocol let alone sub-q vs IM protocol they are following the steps needed in order to make a fair comparison.
Critical Points
1. The protocol needs to be kept the same (ester/dose T/injection frequency)
2. 4-6 weeks for blood levels to stabilize before getting blood work done (6 weeks)
3. Testing is done at the true trough
4. Using the same lab
5. Using the same assays (most accurate) TT/e2 (LC-MS/MS) and FT (Equilibrium Dialysis or Ultrafiltration)
6. Each protocol needs to be given 12 weeks (claim success or failure)
Only then can one make the claim whether the protocol was truly a success or failure let alone when comparing blood levels.