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Guess this went over your noggin too!

* Limitations of using free testosterone by equilibrium dialysis and calculated free testosterone concentrations in practice are the lack of assay standardization, an accuracy-based quality control program, and a harmonized reference range. Until these limitations are addressed, free testosterone by equilibrium dialysis and calculated free testosterone should use reference ranges established by individual laboratories or their specific assay method

Again for the last time!

My reply to previous threads:

When it comes to blood work whether one is following a strictly sub-q protocol, strictly IM protocol let alone sub-q vs IM protocol they are following the steps needed in order to make a fair comparison.

Critical Points

1. The protocol needs to be kept the same (ester/dose T/injection frequency)

2. 4-6 weeks for blood levels to stabilize before getting blood work done (6 weeks)

3. Testing is done at the true trough

4. Using the same lab

5. Using the same assays (most accurate) TT/e2 (LC-MS/MS) and FT (Equilibrium Dialysis or Ultrafiltration)

6. Each protocol needs to be given 12 weeks (claim success or failure)

Only then can one make the claim whether the protocol was truly a success or failure let alone when comparing blood levels.

<blockquote data-quote="madman" data-source="post: 223455" data-attributes="member: 13851">Guess this went over your noggin too!* Limitations of using free testosterone by equilibrium dialysis and calculated free testosterone concentrations in practice are the lack of assay standardization, an accuracy-based quality control program, and a harmonized reference range. Until these limitations are addressed, free testosterone by equilibrium dialysis and calculated free testosterone should use reference ranges established by individual laboratories or their specific assay methodAgain for the last time!My reply to previous threads:When it comes to blood work whether one is following a strictly sub-q protocol, strictly IM protocol let alone sub-q vs IM protocol they are following the steps needed in order to make a fair comparison.Critical Points1. The protocol needs to be kept the same (ester/dose T/injection frequency)2. 4-6 weeks for blood levels to stabilize before getting blood work done (6 weeks)3. Testing is done at the true trough4. Using the same lab5. Using the same assays (most accurate) TT/e2 (LC-MS/MS) and FT (Equilibrium Dialysis or Ultrafiltration)6. Each protocol needs to be given 12 weeks (claim success or failure)Only then can one make the claim whether the protocol was truly a success or failure let alone when comparing blood levels.</blockquote>

[QUOTE="madman, post: 223455, member: 13851"] Guess this went over your noggin too! [B][I]* Limitations of using free testosterone by equilibrium dialysis and calculated free testosterone concentrations in practice are the lack of assay standardization, an accuracy-based quality control program, and a harmonized reference range. [U]Until these limitations are addressed, free testosterone by equilibrium dialysis and calculated free testosterone should use reference ranges established by individual laboratories or their specific assay method[/U][/I][/B] Again for the last time! My reply to previous threads: When it comes to[I] [B]blood work[/B] [/I]whether one is following a strictly sub-q protocol, strictly IM protocol let alone sub-q vs IM protocol they are following the steps needed in order to make a fair comparison. [B][U]Critical Points[/U] 1. [I]The protocol needs to be kept the same (ester/dose T/injection frequency)[/I] 2[/B]. [B][I]4-6 weeks for blood levels to stabilize before getting blood work done (6 weeks)[/I] 3.[/B] [B][I]Testing is done at the true trough[/I] 4.[/B] [B][I]Using the same lab[/I] 5.[/B] [B][I]Using the same assays (most accurate) TT/e2 (LC-MS/MS) and FT (Equilibrium Dialysis or Ultrafiltration)[/I] 6.[/B] [B][I]Each protocol needs to be given 12 weeks (claim success or failure)[/I] Only then can one make the claim whether the protocol was truly a success or failure let alone when comparing blood levels.[/B] [/QUOTE]

Verification

TRT Hormone Predictor Widget

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038