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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
defy / blood labs pre trt, thoughts?
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<blockquote data-quote="madman" data-source="post: 244513" data-attributes="member: 13851"><p>Hard to believe you tested TT/estradiol using the most accurate assays (LC/MS-MS) yet did a 360 on one of the most critical markers FT!</p><p></p><p>Go figure!</p><p></p><p></p><p></p><p>One of the highlights from a recent lecture</p><p>[ATTACH=full]28762[/ATTACH]</p><p></p><p><strong>*Results from the American College of Pathologists Proficiency Testing Survey CAP Y-B, 2021</strong></p><p><strong></strong></p><p><strong>*Total number of 34 laboratories with 5 different platforms participated:</strong></p><p><strong></strong></p><p><strong>*1-4: <u>4 commercial IA manufacturers</u>; 5: <u>Mass spectrometry -based-assay</u></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong></strong></p><p><strong>Proficiency Testing survey (2021)</strong></p><p><strong></strong></p><p><strong><em>Here is the graph showing the data from the recent CAP survey on one sample. There are 34 laboratory participants and they are grouped by the different assay platforms used. There are four different immunoassay platforms and the mass spec is grouped into one category.</em></strong></p><p><strong><em></em></strong></p><p><strong><em>So we demonstrated the results for the report from CAP the measured free testosterone concentration at medium, lowest, and highest levels, and the mean with 3 standard deviations if the peer group has sufficient participants.</em></strong></p><p><strong><em></em></strong></p><p><strong><em>So you can see very clearly that is kind of consistent with this finding there is a big difference between the mass spec generated levels versus immunoassay results.</em></strong></p><p><strong><em></em></strong></p><p><strong><em>Here we see up to a <u>tenfold difference with the mass spec results much higher and within the immunoassay group</u> which you can also see on the right side so also the obvious variability is observed among the groups and also within the group.</em></strong></p><p></p><p><em><strong>So for one survey even with this limitation with the sample commutabilities and also it is not an accuracy-based survey but this <u>data still clearly indicate the high variability across methods</u>.</strong></em></p></blockquote><p></p>
[QUOTE="madman, post: 244513, member: 13851"] Hard to believe you tested TT/estradiol using the most accurate assays (LC/MS-MS) yet did a 360 on one of the most critical markers FT! Go figure! One of the highlights from a recent lecture [ATTACH type="full" alt="Screenshot (20246).png"]28762[/ATTACH] [B]*Results from the American College of Pathologists Proficiency Testing Survey CAP Y-B, 2021 *Total number of 34 laboratories with 5 different platforms participated: *1-4: [U]4 commercial IA manufacturers[/U]; 5: [U]Mass spectrometry -based-assay[/U] Proficiency Testing survey (2021) [I]Here is the graph showing the data from the recent CAP survey on one sample. There are 34 laboratory participants and they are grouped by the different assay platforms used. There are four different immunoassay platforms and the mass spec is grouped into one category. So we demonstrated the results for the report from CAP the measured free testosterone concentration at medium, lowest, and highest levels, and the mean with 3 standard deviations if the peer group has sufficient participants. So you can see very clearly that is kind of consistent with this finding there is a big difference between the mass spec generated levels versus immunoassay results. Here we see up to a [U]tenfold difference with the mass spec results much higher and within the immunoassay group[/U] which you can also see on the right side so also the obvious variability is observed among the groups and also within the group.[/I][/B] [I][B]So for one survey even with this limitation with the sample commutabilities and also it is not an accuracy-based survey but this [U]data still clearly indicate the high variability across methods[/U].[/B][/I] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
defy / blood labs pre trt, thoughts?
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