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<blockquote data-quote="ratbag" data-source="post: 85472" data-attributes="member: 972"><p>Micronutirents are not easy to follow. I had my B-12 tested by my PCP at the hospital and the results were: </p><p></p><p>478 (140-700) pmol/L PCP Hospital labs</p><p></p><p>I've been having trouble with hypothyroid symptoms and although my RT3 was good I decided to get the micronutrient testing done by SpectraCell Laboratories to verify and see if anything else was questionable. This is Spectracell's overview of their test proceedure:</p><p></p><p>OVERVIEW OF TEST PROCEDURE</p><p>1. A mixture of lymphocytes is isolated from the blood.</p><p>2. These cells are grown in a defined culture medium containing optimal levels of all essential.</p><p>nutrients necessary to sustain their growth in cell culture.</p><p>3. The T-lymphocytes are stimulated to grow with a mitogen (phytohemagglutinin) and growth is</p><p>measured by the incorporation of tritiated (radioactive) thymidine into the DNA of the cells.</p><p>The growth response under optimal conditions is defined as 100%, and all other growth rates are</p><p>compared to this 100% level of growth.</p><p>For example – we remove vitamin B6 from the medium and stimulate the cells to grow by mitogen</p><p>stimulation. Growth is measured by DNA synthesis and the rate of growth is dependent only upon the</p><p>functional level of vitamin B6 available within the cells to support growth. For Vitamin B6 a growth rate of</p><p>at least 55% of the growth rate observed in the optimal (100%) media is considered normal. Results less</p><p>than 55% are considered to indicate a functional deficiency for Vitamin B6. Each nutrient has a different</p><p>reference range that was established by assaying thousands of apparently healthy individuals.</p><p>BREAKING DOWN THE REPORT</p><p>1. TEST RESULT (% CONTROL)</p><p>This column represents the patient’s growth response in the test media measured by DNA</p><p>synthesis as compared to the optimal growth observed in the 100% media.</p><p>2. FUNCTIONAL ABNORMALS</p><p>An interpretation is provided for those nutrients found to be deficient.</p><p>3. REFERENCE RANGE</p><p>This column represents how this patient’s result compares to thousands of patients previously</p><p>tested. A patient’s result is considered deficient when it is less than the reference</p><p>range.</p><p></p><p>My results are:</p><p>B-12, 16 (<strong>borderline</strong>) Reference Range (greater than) >14%</p><p>Pantothenate, 10 (<strong>borderline</strong>) Reference Range (greater than) >7%</p><p>Vitamin, K2 34 (<strong>borderline</strong>) Reference Range (greater than) >30%</p><p>Manganese, 56 (<strong>borderline</strong>) Reference Range (greater than) >50%</p><p>Fructose Sensitivity, 36 (<strong>borderline</strong>) Reference Range (greater than) >34%</p><p>Biotin 34, (<strong>deficient</strong>) Reference Range (greater than) >34%</p><p></p><p>Ironically the hospital labs were above 50% in range, yet the Spectracell labs claimed I was borderline.</p><p></p><p>I haven't noticed any difference taking more B12 then previously.</p></blockquote><p></p>
[QUOTE="ratbag, post: 85472, member: 972"] Micronutirents are not easy to follow. I had my B-12 tested by my PCP at the hospital and the results were: 478 (140-700) pmol/L PCP Hospital labs I've been having trouble with hypothyroid symptoms and although my RT3 was good I decided to get the micronutrient testing done by SpectraCell Laboratories to verify and see if anything else was questionable. This is Spectracell's overview of their test proceedure: OVERVIEW OF TEST PROCEDURE 1. A mixture of lymphocytes is isolated from the blood. 2. These cells are grown in a defined culture medium containing optimal levels of all essential. nutrients necessary to sustain their growth in cell culture. 3. The T-lymphocytes are stimulated to grow with a mitogen (phytohemagglutinin) and growth is measured by the incorporation of tritiated (radioactive) thymidine into the DNA of the cells. The growth response under optimal conditions is defined as 100%, and all other growth rates are compared to this 100% level of growth. For example – we remove vitamin B6 from the medium and stimulate the cells to grow by mitogen stimulation. Growth is measured by DNA synthesis and the rate of growth is dependent only upon the functional level of vitamin B6 available within the cells to support growth. For Vitamin B6 a growth rate of at least 55% of the growth rate observed in the optimal (100%) media is considered normal. Results less than 55% are considered to indicate a functional deficiency for Vitamin B6. Each nutrient has a different reference range that was established by assaying thousands of apparently healthy individuals. BREAKING DOWN THE REPORT 1. TEST RESULT (% CONTROL) This column represents the patient’s growth response in the test media measured by DNA synthesis as compared to the optimal growth observed in the 100% media. 2. FUNCTIONAL ABNORMALS An interpretation is provided for those nutrients found to be deficient. 3. REFERENCE RANGE This column represents how this patient’s result compares to thousands of patients previously tested. A patient’s result is considered deficient when it is less than the reference range. My results are: B-12, 16 ([B]borderline[/B]) Reference Range (greater than) >14% Pantothenate, 10 ([B]borderline[/B]) Reference Range (greater than) >7% Vitamin, K2 34 ([B]borderline[/B]) Reference Range (greater than) >30% Manganese, 56 ([B]borderline[/B]) Reference Range (greater than) >50% Fructose Sensitivity, 36 ([B]borderline[/B]) Reference Range (greater than) >34% Biotin 34, ([B]deficient[/B]) Reference Range (greater than) >34% Ironically the hospital labs were above 50% in range, yet the Spectracell labs claimed I was borderline. I haven't noticed any difference taking more B12 then previously. [/QUOTE]
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