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ExcelFemale
HRT in Women
Androgen production within the human vagina
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<blockquote data-quote="madman" data-source="post: 191641" data-attributes="member: 13851"><p><strong>Figure 5. <span style="color: rgb(184, 49, 47)">Measurement in mass spectrometry of androstenedione, testosterone, and DHT concentrations by LC-MS/MS in the culture media of hvSMCs in response to stimulation with increasing doses of DHEA </span>Panel A <span style="color: rgb(184, 49, 47)">shows the androstenedione concentrations </span><span style="color: rgb(0, 0, 0)">(expressed as nmol/L) </span><span style="color: rgb(184, 49, 47)">measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA</span><span style="color: rgb(0, 0, 0)"> (from 1 to 30 nM)</span><span style="color: rgb(184, 49, 47)"> at 24 and 48 h.</span> Results are reported as mean±SEM of three independent experiments performed in triplicate. </strong></p><p>[ATTACH=full]11896[/ATTACH]</p><p></p><p></p><p><strong>Panel B <span style="color: rgb(184, 49, 47)">shows the testosterone concentrations </span><span style="color: rgb(0, 0, 0)">(expressed as nmol/l)</span><span style="color: rgb(184, 49, 47)"> measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA </span><span style="color: rgb(0, 0, 0)">(from 1 to 30 nM)</span><span style="color: rgb(184, 49, 47)"> at 24 and 48 h.</span> Results are reported as mean±SEM of three independent experiments performed in triplicate. </strong></p><p>[ATTACH=full]11897[/ATTACH]</p><p></p><p></p><p><strong>Panel C <span style="color: rgb(184, 49, 47)">shows the DHT concentrations </span><span style="color: rgb(0, 0, 0)">(expressed as nmol/l)</span><span style="color: rgb(184, 49, 47)"> measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA </span><span style="color: rgb(0, 0, 0)">(from 1 nM to 1 µM)</span><span style="color: rgb(184, 49, 47)"> and physiological/supraphysiological doses of T </span><span style="color: rgb(0, 0, 0)">(0.6 and 30 nM)</span><span style="color: rgb(184, 49, 47)"> at 48 h. </span>Results are reported as mean±SEM of three independent experiments performed in triplicate. </strong></p><p><strong>°°° p<0.001 vs NT, * p<0.05, *** p<0.001 vs all lower DHEA doses (nM); ^^^ p<0.001 vs T (0.6 nM) </strong></p><p>[ATTACH=full]11898[/ATTACH]</p></blockquote><p></p>
[QUOTE="madman, post: 191641, member: 13851"] [B]Figure 5. [COLOR=rgb(184, 49, 47)]Measurement in mass spectrometry of androstenedione, testosterone, and DHT concentrations by LC-MS/MS in the culture media of hvSMCs in response to stimulation with increasing doses of DHEA [/COLOR]Panel A [COLOR=rgb(184, 49, 47)]shows the androstenedione concentrations [/COLOR][COLOR=rgb(0, 0, 0)](expressed as nmol/L) [/COLOR][COLOR=rgb(184, 49, 47)]measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA[/COLOR][COLOR=rgb(0, 0, 0)] (from 1 to 30 nM)[/COLOR][COLOR=rgb(184, 49, 47)] at 24 and 48 h.[/COLOR] Results are reported as mean±SEM of three independent experiments performed in triplicate. [/B] [ATTACH type="full"]11896[/ATTACH] [B]Panel B [COLOR=rgb(184, 49, 47)]shows the testosterone concentrations [/COLOR][COLOR=rgb(0, 0, 0)](expressed as nmol/l)[/COLOR][COLOR=rgb(184, 49, 47)] measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA [/COLOR][COLOR=rgb(0, 0, 0)](from 1 to 30 nM)[/COLOR][COLOR=rgb(184, 49, 47)] at 24 and 48 h.[/COLOR] Results are reported as mean±SEM of three independent experiments performed in triplicate. [/B] [ATTACH type="full"]11897[/ATTACH] [B]Panel C [COLOR=rgb(184, 49, 47)]shows the DHT concentrations [/COLOR][COLOR=rgb(0, 0, 0)](expressed as nmol/l)[/COLOR][COLOR=rgb(184, 49, 47)] measured in the culture media of hvSMCs after stimulation with increasing doses of DHEA [/COLOR][COLOR=rgb(0, 0, 0)](from 1 nM to 1 µM)[/COLOR][COLOR=rgb(184, 49, 47)] and physiological/supraphysiological doses of T [/COLOR][COLOR=rgb(0, 0, 0)](0.6 and 30 nM)[/COLOR][COLOR=rgb(184, 49, 47)] at 48 h. [/COLOR]Results are reported as mean±SEM of three independent experiments performed in triplicate. °°° p<0.001 vs NT, * p<0.05, *** p<0.001 vs all lower DHEA doses (nM); ^^^ p<0.001 vs T (0.6 nM) [/B] [ATTACH type="full"]11898[/ATTACH] [/QUOTE]
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ExcelFemale
HRT in Women
Androgen production within the human vagina
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