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ExcelFemale
HRT in Women
Androgen production within the human vagina
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<blockquote data-quote="madman" data-source="post: 191638" data-attributes="member: 13851"><p><strong>Figure 2. <span style="color: rgb(184, 49, 47)">Relative mRNA expression of sex steroid receptors in human distal vaginal tissues and cells </span><span style="color: rgb(0, 0, 0)">Panels A and B </span><span style="color: rgb(184, 49, 47)">show the relative mRNA expression of sex steroid receptors AR, ERα, ERβ, GPER1, and PR in human vaginal tissues derived from laparoscopic biopsies </span><span style="color: rgb(0, 0, 0)">(n=10)</span><span style="color: rgb(184, 49, 47)"> and in smooth muscle cells </span><span style="color: rgb(0, 0, 0)">(hSMCs)</span><span style="color: rgb(184, 49, 47)"> isolated from the corresponding vaginal samples </span><span style="color: rgb(0, 0, 0)">(n=4, performed in triplicate)</span><span style="color: rgb(184, 49, 47)">, respectively. </span>Data were calculated according to the 2-ΔΔCt comparative method, using ribosomal subunit 18S as the housekeeping gene for normalization. Results are expressed as arbitrary units, calculated as fold-increase vs. the lower mRNA expression value in the experiment, and are reported as mean±SEM. </strong></p><p><strong>* p<0.05, *** p<0.001 vs AR; °°° p<0.001 vs ERα; ## p<0.01, ### p<0.001 vs ERβ; ++ p<0.01, +++ p<0.001 vs GPER1</strong></p><p>[ATTACH=full]11891[/ATTACH]</p><p>[ATTACH=full]11892[/ATTACH]</p><p><strong>Panels C and D <span style="color: rgb(184, 49, 47)">show two representative images of nuclear immunostaining for AR receptor and the corresponding nuclear DAPI double labeling, respectively. </span>Panel E <span style="color: rgb(184, 49, 47)">shows a representative image of the negative control obtained omitting the primary antibodies. </span>At least three preparations obtained from three different patients were evaluated (scale bar=100 µm).</strong></p></blockquote><p></p>
[QUOTE="madman, post: 191638, member: 13851"] [B]Figure 2. [COLOR=rgb(184, 49, 47)]Relative mRNA expression of sex steroid receptors in human distal vaginal tissues and cells [/COLOR][COLOR=rgb(0, 0, 0)]Panels A and B [/COLOR][COLOR=rgb(184, 49, 47)]show the relative mRNA expression of sex steroid receptors AR, ERα, ERβ, GPER1, and PR in human vaginal tissues derived from laparoscopic biopsies [/COLOR][COLOR=rgb(0, 0, 0)](n=10)[/COLOR][COLOR=rgb(184, 49, 47)] and in smooth muscle cells [/COLOR][COLOR=rgb(0, 0, 0)](hSMCs)[/COLOR][COLOR=rgb(184, 49, 47)] isolated from the corresponding vaginal samples [/COLOR][COLOR=rgb(0, 0, 0)](n=4, performed in triplicate)[/COLOR][COLOR=rgb(184, 49, 47)], respectively. [/COLOR]Data were calculated according to the 2-ΔΔCt comparative method, using ribosomal subunit 18S as the housekeeping gene for normalization. Results are expressed as arbitrary units, calculated as fold-increase vs. the lower mRNA expression value in the experiment, and are reported as mean±SEM. * p<0.05, *** p<0.001 vs AR; °°° p<0.001 vs ERα; ## p<0.01, ### p<0.001 vs ERβ; ++ p<0.01, +++ p<0.001 vs GPER1[/B] [ATTACH type="full" alt="Screenshot (2813).png"]11891[/ATTACH] [ATTACH type="full" alt="Screenshot (2814).png"]11892[/ATTACH] [B]Panels C and D [COLOR=rgb(184, 49, 47)]show two representative images of nuclear immunostaining for AR receptor and the corresponding nuclear DAPI double labeling, respectively. [/COLOR]Panel E [COLOR=rgb(184, 49, 47)]shows a representative image of the negative control obtained omitting the primary antibodies. [/COLOR]At least three preparations obtained from three different patients were evaluated (scale bar=100 µm).[/B] [/QUOTE]
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ExcelFemale
HRT in Women
Androgen production within the human vagina
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