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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
A High-throughput Method for Accurate Measurement of FT in Serum using ED Coupled with ID-UHPLC-MS/MS
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<blockquote data-quote="madman" data-source="post: 270815" data-attributes="member: 13851"><p><strong>Introduction</strong></p><p></p><p><em>Testosterone is the most abundant circulating androgen in both men and women. It is a biomarker for identifying androgen excess and deficiency, and to monitor their treatment. <strong>Measurement of the metabolically active form, free testosterone (FT), has been part of the clinical workup, especially in individuals with impaired sex hormone binding globulin (SHBG) levels[1]. </strong>FT is currently measured using direct commercial immunoassays, assays using equilibrium dialysis (ED), or other separation techniques<strong>. Though the ED approach is recognized as the recommended approach, it is laborious and technically challenging for routine clinical use. The CDC Clinical Standardization Program is developing a high throughput method using the ED procedure coupled with isotope dilution ultra-high-performance liquid chromatography-tandem mass spectrometry (ID-UHPLC-MS/MS) that can be used in patient care, public health, and clinical research.</strong></em></p><p><em><strong></strong></em></p><p><em><strong></strong></em></p><p><em><strong></strong></em></p><p><em><strong></strong></em></p><p><em><strong>Nov2/2022 (original thread)</strong></em></p><p>[URL unfurl="true"]https://www.excelmale.com/forum/threads/measurement-of-ft-in-serum-using-ed-coupled-with-id-uhplc-ms-ms-in-comparison-to-cftv.26364/[/URL]</p></blockquote><p></p>
[QUOTE="madman, post: 270815, member: 13851"] [B]Introduction[/B] [I]Testosterone is the most abundant circulating androgen in both men and women. It is a biomarker for identifying androgen excess and deficiency, and to monitor their treatment. [B]Measurement of the metabolically active form, free testosterone (FT), has been part of the clinical workup, especially in individuals with impaired sex hormone binding globulin (SHBG) levels[1]. [/B]FT is currently measured using direct commercial immunoassays, assays using equilibrium dialysis (ED), or other separation techniques[B]. Though the ED approach is recognized as the recommended approach, it is laborious and technically challenging for routine clinical use. The CDC Clinical Standardization Program is developing a high throughput method using the ED procedure coupled with isotope dilution ultra-high-performance liquid chromatography-tandem mass spectrometry (ID-UHPLC-MS/MS) that can be used in patient care, public health, and clinical research. Nov2/2022 (original thread)[/B][/I] [URL unfurl="true"]https://www.excelmale.com/forum/threads/measurement-of-ft-in-serum-using-ed-coupled-with-id-uhplc-ms-ms-in-comparison-to-cftv.26364/[/URL] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone and Men's Health Articles
A High-throughput Method for Accurate Measurement of FT in Serum using ED Coupled with ID-UHPLC-MS/MS
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