tt/ft assay; testing; hypogonadism; lse

Analysis of Direct Free and Total Testosterone Using a Single LC-MS/MS Method and Supported Liquid Extraction Procedure

MSACL 2025 Abstract Leslie Farris, Sarah Zilka, Katie Troike, Jena Talbot, Richard Giles, Drew Payto, Jessica M Colón-Franco Department of Laboratory Medicine, Cleveland Clinic Foundation, Cleveland, OH, USA Abstract OBJECTIVE(S) To develop and validate a single LC-MS/MS method for the...
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TRT Hormone Predictor Widget

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038