hypogonadism; free testosterone; ed assay

Optimized state-of-the-art direct equilibrium dialysis for free testosterone analysis by LC-MS/MS: Achieving accurate measurements in hours

* Equilibrium dialysis (ED) is the preferred method for measuring FT, followed by ultrafiltration, but these methods are technically challenging and time-consuming. Calculation of FT from total testosterone and SHBG (with or without albumin) has limitations and may be inaccurate. There is a...
- madman
- Thread
- Jul 30, 2025
- hypogonadism; free testosterone; ed assay
- Replies: 2
- Forum: Testosterone and Men's Health Articles

TRT Hormone Predictor Widget

Understanding Your Hormones

Estradiol (E2)

A form of estrogen produced from testosterone. Important for bone health, mood, and libido. Too high can cause side effects; too low can affect well-being.

DHT

Dihydrotestosterone is a potent androgen derived from testosterone. Affects hair growth, prostate health, and masculinization effects.

Free Testosterone

The biologically active form of testosterone not bound to proteins. Directly available for cellular uptake and biological effects.

Scientific Reference

Lakshman KM, Kaplan B, Travison TG, Basaria S, Knapp PE, Singh AB, LaValley MP, Mazer NA, Bhasin S. The effects of injected testosterone dose and age on the conversion of testosterone to estradiol and dihydrotestosterone in young and older men. J Clin Endocrinol Metab. 2010 Aug;95(8):3955-64.

DOI: 10.1210/jc.2010-0102 | PMID: 20534765 | PMCID: PMC2913038