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I reconstitute the 5/5 mg IPA/CJC with 2.5 ml of BAC. That yields 2 mg/ml or 200 mcg/10 units or 0.1 cc. Usual dosing is 100 - 300 mcg before bed. Always start with the lowest dose. You should feel a flushing sensation within minutes after a sc inj. PS products have a vacuum in the vial. Be careful to slowly let the BAC in or you'll foam and possibly denature the product. Mixed and refrigerated, 30 day shelf life.