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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Calculate Free Testosterone with TruT by FPT
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<blockquote data-quote="madman" data-source="post: 145382" data-attributes="member: 13851"><p><strong>[ATTACH=full]7259[/ATTACH]</strong></p><p><strong><span style="color: rgb(184, 49, 47)">[0026] Fig. 7</span> depicts <span style="color: rgb(184, 49, 47)">the</span> <span style="color: rgb(184, 49, 47)">thermodynamic Parameters</span> associated with <span style="color: rgb(184, 49, 47)">testosterone's binding to SHBG</span> derived from the <span style="color: rgb(184, 49, 47)">fit of binding isotherms and ITC data to new model.</span> While this parameter set is not unique, together they consistently describe the <span style="color: rgb(41, 105, 176)">binding isotherms, depletion curves and ITC data </span><span style="color: rgb(0, 0, 0)">to the </span><span style="color: rgb(41, 105, 176)">new Multi-step Dynamic Binding Model with Complex Allostery developed in this study. </span>These were utilized to obtain <span style="color: rgb(41, 105, 176)">FT values (cFTZBJ)</span> in samples obtained in clinical trials.</strong></p><p></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7260[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">[0027] Figs. 8A-8B</span> demonstrate that <span style="color: rgb(184, 49, 47)">the</span> <span style="color: rgb(184, 49, 47)">binding of testosterone to SHBG </span>displays <span style="color: rgb(184, 49, 47)">complex allostery.</span></strong> <strong>Fig. 8A depicts a graph demonstrating that <span style="color: rgb(184, 49, 47)">binding isotherms </span>display <span style="color: rgb(184, 49, 47)">significant non-linearity.</span> Varying concentrations of testosterone were incubated with a <span style="color: rgb(184, 49, 47)">fixed concentration of SHBG (5, 10 or 20 nM)</span> and <span style="color: rgb(184, 49, 47)">bound testosterone </span>was plotted against <span style="color: rgb(184, 49, 47)">total testosterone concentration. </span>The binding isotherms were generated at 5, 10 and 20 nM SHBG.</strong> <strong><span style="color: rgb(184, 49, 47)">Curves represent the result of the fit of data to the new Multi-step Dynamic Binding Model with Complex Allostery.</span></strong></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7261[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">Fig. 8B </span>depicts a graph demonstrating that <span style="color: rgb(41, 105, 176)">depletion of FT by varying SHBG concentration </span>is best described by the <span style="color: rgb(41, 105, 176)">new Multi-step Dynamic Binding Model with Complex Allostery. </span>Constant concentration of testosterone (6, 12 , 17 or 32 nM) was incubated with <span style="color: rgb(41, 105, 176)">increasing SHBG concentrations</span>, and free testosterone concentration in buffer side was plotted against <span style="color: rgb(41, 105, 176)">SHBG concentration.</span> The curves are the result of the fit of data to the <span style="color: rgb(41, 105, 176)">new Multi-step Dynamic Binding Model with Complex Allostery.</span></strong></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7262[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">[0028] Figs. 9A-9C </span>depicts graphs depicting <span style="color: rgb(184, 49, 47)">the fits of the various models of testosterone's binding to SHBG </span>to the experimental data from<span style="color: rgb(184, 49, 47)"> binding isotherms, depletion experiments, and ITC.</span> Left panels: <span style="color: rgb(41, 105, 176)">The figures show the fits of data to the various models examined in this study.</span>Right panels: <span style="color: rgb(41, 105, 176)">The figures show corresponding residuals of the fit of data to various models of testosterone's binding to SHBG.</span> Neither the <span style="color: rgb(184, 49, 47)">Vermeulen's equation </span>nor the <span style="color: rgb(184, 49, 47)">simple allostery models </span>adequately fit the experimental data from <span style="color: rgb(184, 49, 47)">binding isotherms, depletion experiments, or ITC.</span> <span style="color: rgb(41, 105, 176)">The new Multi-step Dynamic Binding Model with Complex Allostery (model E) </span><span style="color: rgb(0, 0, 0)">provided the </span><span style="color: rgb(41, 105, 176)">optimal fit </span><span style="color: rgb(0, 0, 0)">to the experimental data from all three methods.</span></strong></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7263[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">[0029] Fig. 10</span> depicts <span style="color: rgb(184, 49, 47)">a schematic of the</span> <span style="color: rgb(184, 49, 47)">control of testosterone levels.</span></strong></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7264[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">[0030] Fig. 11</span> depicts <span style="color: rgb(184, 49, 47)">a</span> <span style="color: rgb(184, 49, 47)">schematic of an exemplary system of determining free testosterone levels and/or dosages.</span></strong></p><p></p><p></p><p></p><p></p><p>[ATTACH=full]7265[/ATTACH]</p><p><strong><span style="color: rgb(184, 49, 47)">[0031] Fig. 12 </span>depicts <span style="color: rgb(184, 49, 47)">a</span> <span style="color: rgb(184, 49, 47)">device or a computer system </span><span style="color: rgb(41, 105, 176)">1000 </span><span style="color: rgb(184, 49, 47)">comprising one or more processors </span><span style="color: rgb(41, 105, 176)">1300</span><span style="color: rgb(184, 49, 47)"> and a memory </span><span style="color: rgb(41, 105, 176)">1500</span><span style="color: rgb(184, 49, 47)"> storing one or more programs </span><span style="color: rgb(41, 105, 176)">1600 </span><span style="color: rgb(184, 49, 47)">for execution by the one or more processors </span><span style="color: rgb(41, 105, 176)">1300</span><span style="color: rgb(184, 49, 47)">.</span></strong></p></blockquote><p></p>
[QUOTE="madman, post: 145382, member: 13851"] [B][ATTACH=full]7259[/ATTACH] [COLOR=rgb(184, 49, 47)][0026] Fig. 7[/COLOR] depicts [COLOR=rgb(184, 49, 47)]the[/COLOR] [COLOR=rgb(184, 49, 47)]thermodynamic Parameters[/COLOR] associated with [COLOR=rgb(184, 49, 47)]testosterone's binding to SHBG[/COLOR] derived from the [COLOR=rgb(184, 49, 47)]fit of binding isotherms and ITC data to new model.[/COLOR] While this parameter set is not unique, together they consistently describe the [COLOR=rgb(41, 105, 176)]binding isotherms, depletion curves and ITC data [/COLOR][COLOR=rgb(0, 0, 0)]to the [/COLOR][COLOR=rgb(41, 105, 176)]new Multi-step Dynamic Binding Model with Complex Allostery developed in this study. [/COLOR]These were utilized to obtain [COLOR=rgb(41, 105, 176)]FT values (cFTZBJ)[/COLOR] in samples obtained in clinical trials.[/B] [ATTACH=full]7260[/ATTACH] [B][COLOR=rgb(184, 49, 47)][0027] Figs. 8A-8B[/COLOR] demonstrate that [COLOR=rgb(184, 49, 47)]the[/COLOR] [COLOR=rgb(184, 49, 47)]binding of testosterone to SHBG [/COLOR]displays [COLOR=rgb(184, 49, 47)]complex allostery.[/COLOR][/B] [B]Fig. 8A depicts a graph demonstrating that [COLOR=rgb(184, 49, 47)]binding isotherms [/COLOR]display [COLOR=rgb(184, 49, 47)]significant non-linearity.[/COLOR] Varying concentrations of testosterone were incubated with a [COLOR=rgb(184, 49, 47)]fixed concentration of SHBG (5, 10 or 20 nM)[/COLOR] and [COLOR=rgb(184, 49, 47)]bound testosterone [/COLOR]was plotted against [COLOR=rgb(184, 49, 47)]total testosterone concentration. [/COLOR]The binding isotherms were generated at 5, 10 and 20 nM SHBG.[/B] [B][COLOR=rgb(184, 49, 47)]Curves represent the result of the fit of data to the new Multi-step Dynamic Binding Model with Complex Allostery.[/COLOR][/B] [ATTACH=full]7261[/ATTACH] [B][COLOR=rgb(184, 49, 47)]Fig. 8B [/COLOR]depicts a graph demonstrating that [COLOR=rgb(41, 105, 176)]depletion of FT by varying SHBG concentration [/COLOR]is best described by the [COLOR=rgb(41, 105, 176)]new Multi-step Dynamic Binding Model with Complex Allostery. [/COLOR]Constant concentration of testosterone (6, 12 , 17 or 32 nM) was incubated with [COLOR=rgb(41, 105, 176)]increasing SHBG concentrations[/COLOR], and free testosterone concentration in buffer side was plotted against [COLOR=rgb(41, 105, 176)]SHBG concentration.[/COLOR] The curves are the result of the fit of data to the [COLOR=rgb(41, 105, 176)]new Multi-step Dynamic Binding Model with Complex Allostery.[/COLOR][/B] [ATTACH=full]7262[/ATTACH] [B][COLOR=rgb(184, 49, 47)][0028] Figs. 9A-9C [/COLOR]depicts graphs depicting [COLOR=rgb(184, 49, 47)]the fits of the various models of testosterone's binding to SHBG [/COLOR]to the experimental data from[COLOR=rgb(184, 49, 47)] binding isotherms, depletion experiments, and ITC.[/COLOR] Left panels: [COLOR=rgb(41, 105, 176)]The figures show the fits of data to the various models examined in this study.[/COLOR]Right panels: [COLOR=rgb(41, 105, 176)]The figures show corresponding residuals of the fit of data to various models of testosterone's binding to SHBG.[/COLOR] Neither the [COLOR=rgb(184, 49, 47)]Vermeulen's equation [/COLOR]nor the [COLOR=rgb(184, 49, 47)]simple allostery models [/COLOR]adequately fit the experimental data from [COLOR=rgb(184, 49, 47)]binding isotherms, depletion experiments, or ITC.[/COLOR] [COLOR=rgb(41, 105, 176)]The new Multi-step Dynamic Binding Model with Complex Allostery (model E) [/COLOR][COLOR=rgb(0, 0, 0)]provided the [/COLOR][COLOR=rgb(41, 105, 176)]optimal fit [/COLOR][COLOR=rgb(0, 0, 0)]to the experimental data from all three methods.[/COLOR][/B] [ATTACH=full]7263[/ATTACH] [B][COLOR=rgb(184, 49, 47)][0029] Fig. 10[/COLOR] depicts [COLOR=rgb(184, 49, 47)]a schematic of the[/COLOR] [COLOR=rgb(184, 49, 47)]control of testosterone levels.[/COLOR][/B] [ATTACH=full]7264[/ATTACH] [B][COLOR=rgb(184, 49, 47)][0030] Fig. 11[/COLOR] depicts [COLOR=rgb(184, 49, 47)]a[/COLOR] [COLOR=rgb(184, 49, 47)]schematic of an exemplary system of determining free testosterone levels and/or dosages.[/COLOR][/B] [ATTACH=full]7265[/ATTACH] [B][COLOR=rgb(184, 49, 47)][0031] Fig. 12 [/COLOR]depicts [COLOR=rgb(184, 49, 47)]a[/COLOR] [COLOR=rgb(184, 49, 47)]device or a computer system [/COLOR][COLOR=rgb(41, 105, 176)]1000 [/COLOR][COLOR=rgb(184, 49, 47)]comprising one or more processors [/COLOR][COLOR=rgb(41, 105, 176)]1300[/COLOR][COLOR=rgb(184, 49, 47)] and a memory [/COLOR][COLOR=rgb(41, 105, 176)]1500[/COLOR][COLOR=rgb(184, 49, 47)] storing one or more programs [/COLOR][COLOR=rgb(41, 105, 176)]1600 [/COLOR][COLOR=rgb(184, 49, 47)]for execution by the one or more processors [/COLOR][COLOR=rgb(41, 105, 176)]1300[/COLOR][COLOR=rgb(184, 49, 47)].[/COLOR][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
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Calculate Free Testosterone with TruT by FPT
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