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Message: <blockquote data-quote="4:15amstreet" data-source="post: 185433" data-attributes="member: 41174">Blood spot testing is all over the place for accuracy. I would not trust it, especially with respect to Estradiol. I found this article <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2175208/" target="_blank">A comparison of blood spot versus plasma analysis of gonadotropin and ovarian steroid hormone levels in reproductive-age women</a>where the owner of ZRT labs admits that it is not accurate for estradiol. It is sometimes off by a factor of 5! I personally did a test with ZRT and the results suggested I could benefit from TRT, but I wanted to be sure, so I did a blood draw test. My total testosterone was over twice what they said it was in the course of a month with no supplementation. Don't rely on them at all.</blockquote>