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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Direct Measurements of Free Hormones Using Mass Spectrometry-Based Methods
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<blockquote data-quote="madman" data-source="post: 260328" data-attributes="member: 13851"><p><strong>Fig. 1. Analysis of free hormone concentrations involves several basic steps. The first step of sample preparation involves separation of the free analyte of interest from protein-bound analyte. This can be done by size-exclusion (SE), equilibrium dialysis (ED), or ultrafiltration (UF). Next, internal standard (IS, stable isotope labeled analog of the FH of interest) is added to the samples, the isolated free analyte and the IS are extracted from the biological matrix, and the extracted free analyte and the IS are separated by liquid chromatography and detected by mass spectrometry. This figure was created with BioRender.com.</strong></p><p><strong>[ATTACH=full]34925[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 260328, member: 13851"] [B]Fig. 1. Analysis of free hormone concentrations involves several basic steps. The first step of sample preparation involves separation of the free analyte of interest from protein-bound analyte. This can be done by size-exclusion (SE), equilibrium dialysis (ED), or ultrafiltration (UF). Next, internal standard (IS, stable isotope labeled analog of the FH of interest) is added to the samples, the isolated free analyte and the IS are extracted from the biological matrix, and the extracted free analyte and the IS are separated by liquid chromatography and detected by mass spectrometry. This figure was created with BioRender.com. [ATTACH type="full"]34925[/ATTACH][/B] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Blood Test Discussion
Direct Measurements of Free Hormones Using Mass Spectrometry-Based Methods
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