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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Very high TT on low dose TRT
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<blockquote data-quote="tareload" data-source="post: 226849"><p>Run LC/MS-MS for TT.</p><p></p><p>Example:</p><p>[URL unfurl="true"]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/[/URL]</p><p></p><p>...</p><p><em>Some methods described in literature use derivatization procedures to enable analysis by GC-MS or to achieve higher sensitivity of LC-MS measurements [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R47" target="_blank">47</a>–<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R49" target="_blank">49</a>]. Our method does not require derivatization, which minimizes complexity and facilitates automation. Furthermore, other methods employ ethers, such as methyl tertiary-butyl ether [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R24" target="_blank">24</a>], diethyl ether [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R50" target="_blank">50</a>], and diethyl ether/ethyl acetate mixtures [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R51" target="_blank">51</a>] for the liquid–liquid extraction. However, these solvents are difficult to handle with automatic pipetting system and require special safety considerations. The solvents used in our method showed compatibility with automated liquid handlers and require fewer safety considerations than ethers [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R52" target="_blank">52</a>–<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R54" target="_blank">54</a>].</em></p><p><em></em></p><p><em>The present method proofed a consistent result over a 2-year period at all concentration levels, as well as experimental variations with very minimal matrix effect and no or negligible difference for testosterone in neat and matrix. Measurement values obtained with this method are in high agreement with established reference methods [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R30" target="_blank">30</a>] and meets performance criteria for accuracy used by the CDC Hormone Standardization Program [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R35" target="_blank">35</a>]. This described method provides a consistently high accurate and sensitive LC-MS/MS assay for testosterone measurements in serum at all concentration levels typically observed in men as well as in women and children.</em></p></blockquote><p></p>
[QUOTE="tareload, post: 226849"] Run LC/MS-MS for TT. Example: [URL unfurl="true"]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/[/URL] ... [I]Some methods described in literature use derivatization procedures to enable analysis by GC-MS or to achieve higher sensitivity of LC-MS measurements [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R47']47[/URL]–[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R49']49[/URL]]. Our method does not require derivatization, which minimizes complexity and facilitates automation. Furthermore, other methods employ ethers, such as methyl tertiary-butyl ether [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R24']24[/URL]], diethyl ether [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R50']50[/URL]], and diethyl ether/ethyl acetate mixtures [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R51']51[/URL]] for the liquid–liquid extraction. However, these solvents are difficult to handle with automatic pipetting system and require special safety considerations. The solvents used in our method showed compatibility with automated liquid handlers and require fewer safety considerations than ethers [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R52']52[/URL]–[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R54']54[/URL]]. The present method proofed a consistent result over a 2-year period at all concentration levels, as well as experimental variations with very minimal matrix effect and no or negligible difference for testosterone in neat and matrix. Measurement values obtained with this method are in high agreement with established reference methods [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R30']30[/URL]] and meets performance criteria for accuracy used by the CDC Hormone Standardization Program [[URL='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743493/#R35']35[/URL]]. This described method provides a consistently high accurate and sensitive LC-MS/MS assay for testosterone measurements in serum at all concentration levels typically observed in men as well as in women and children.[/I] [/QUOTE]
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Testosterone Replacement, Low T, HCG, & Beyond
Testosterone Basics & Questions
Very high TT on low dose TRT
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