ExcelMale
Menu
Home
What's new
Latest activity
Forums
New posts
Search forums
What's new
New posts
Latest activity
Videos
Lab Tests
Doctor Finder
Buy Books
About Us
Men’s Health Coaching
Log in
Register
What's new
Search
Search
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Navigation
Install the app
Install
More options
Contact us
Close Menu
Forums
General Health & Fitness
Nutrition and Supplements
Understanding the effects of nutrition and post-exercise nutrition on skeletal muscle protein turnover
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Message
<blockquote data-quote="madman" data-source="post: 208305" data-attributes="member: 13851"><p><strong>Fig. 1. <u>Schematic representation of the use of AA stable isotope tracers and D2O for the measurement of muscle protein turnover</u>. (A) To measure acute (typically 0e6 h post-exercise and/or feeding) MPS, stable AA isotope tracers are infused intravenously which results in an increased plasma enrichment of the isotope. The AA isotope is taken up by the muscle (rate of disappearance) into the intracellular AA pool. The labeled AA is incorporated into new proteins (MPS). As infusion time progresses the change in incorporated AA stable isotope is proportionate to the rate of total MPS. Alternatively, MPB can be measured through the release of labeled AA stable isotope into the intracellular AA pool or inferred from the rate of appearance of the labeled AA into the venous blood samples. (B) To measure integrated (free-living) MPS D2O is ingested and results in an increase in the enrichment of the body water pool. Once entering the cell the deuterium becomes incorporated into alanine during the transamination process, these deuterium-labeled alanine AA are then incorporated into new muscle proteins and are indicative of MPS. (C) For both methods, muscle protein/plasma/saliva enrichment is determined using mass spectrometry following derivatization of AA. To calculate the rate of MPS, the enrichment of one muscle sample (T1) is subtracted from the enrichment of a later muscle sample (T2) and divided by the mean enrichment of the precursor (plasma enrichment when using stable AA isotope; body water enrichment when using D2O) multiplied by the time.</strong></p><p><strong>[ATTACH=full]16479[/ATTACH]</strong></p></blockquote><p></p>
[QUOTE="madman, post: 208305, member: 13851"] [B]Fig. 1. [U]Schematic representation of the use of AA stable isotope tracers and D2O for the measurement of muscle protein turnover[/U]. (A) To measure acute (typically 0e6 h post-exercise and/or feeding) MPS, stable AA isotope tracers are infused intravenously which results in an increased plasma enrichment of the isotope. The AA isotope is taken up by the muscle (rate of disappearance) into the intracellular AA pool. The labeled AA is incorporated into new proteins (MPS). As infusion time progresses the change in incorporated AA stable isotope is proportionate to the rate of total MPS. Alternatively, MPB can be measured through the release of labeled AA stable isotope into the intracellular AA pool or inferred from the rate of appearance of the labeled AA into the venous blood samples. (B) To measure integrated (free-living) MPS D2O is ingested and results in an increase in the enrichment of the body water pool. Once entering the cell the deuterium becomes incorporated into alanine during the transamination process, these deuterium-labeled alanine AA are then incorporated into new muscle proteins and are indicative of MPS. (C) For both methods, muscle protein/plasma/saliva enrichment is determined using mass spectrometry following derivatization of AA. To calculate the rate of MPS, the enrichment of one muscle sample (T1) is subtracted from the enrichment of a later muscle sample (T2) and divided by the mean enrichment of the precursor (plasma enrichment when using stable AA isotope; body water enrichment when using D2O) multiplied by the time. [ATTACH type="full"]16479[/ATTACH][/B] [/QUOTE]
Insert quotes…
Verification
Post reply
Share this page
Facebook
Twitter
Reddit
Pinterest
Tumblr
WhatsApp
Email
Share
Link
Sponsors
Forums
General Health & Fitness
Nutrition and Supplements
Understanding the effects of nutrition and post-exercise nutrition on skeletal muscle protein turnover
This site uses cookies to help personalise content, tailor your experience and to keep you logged in if you register.
By continuing to use this site, you are consenting to our use of cookies.
Accept
Learn more…
Top